Difference between revisions of "Part:BBa K515104"
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<p><b>This BioBrick has been sequence verified.</p></b>
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<p>This BioBrick has been sequence verified. It has also been tested for protein expression. A nice clear band of the appropriate size can be seen at the bottom of the gel indicating a strong over-expression of a small protein of the appropriate size.</p>
  
 
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Revision as of 02:19, 22 September 2011

J23100 promoter - Antiholin

A composite part between J23100 and anti-holin (BBa_K515004). The promoter has been chosen in order to comply with our Gene Guard device specifications. It is under the influence of a strong promoter due to the lower copy number of this gene (will be integrated into the genome) in comparison to that of the Holin gene which will be in a high copy number plasmid within our system.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 276


This BioBrick has been sequence verified. It has also been tested for protein expression. A nice clear band of the appropriate size can be seen at the bottom of the gel indicating a strong over-expression of a small protein of the appropriate size.

Figure 1: Protein gel showing an over-expression band (higher in intensity) of a small protein. Lane 1 and 2 are control and lane 3 and 4 are the anti-holin expressing cells.(Data by Imperial College London iGEM team 2011).