Difference between revisions of "Part:BBa K676006:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | Design primers for the PCR to extract the DNA sequence as well as primers for the site directed mutagenesis to remove the XbaI restriction site at the 241th bp position in the 892bp T5 exonuclease DNA sequence. | |
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===Source=== | ===Source=== | ||
Revision as of 02:11, 22 September 2011
T5 Phage Exonuclease
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 600
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Design primers for the PCR to extract the DNA sequence as well as primers for the site directed mutagenesis to remove the XbaI restriction site at the 241th bp position in the 892bp T5 exonuclease DNA sequence.
Source
T5 Bacteriophage