Difference between revisions of "Part:BBa K564013"
 
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<partinfo>BBa_K564013 short</partinfo>
 
<partinfo>BBa_K564013 short</partinfo>
  
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ChiP encodes enterobacterial chitoporin required for uptake of chitin-derived oligosaccharides. This outer membrane porin is required for Salmonella and E.coli growth on chitooligosaccharides as a sole source of both carbon and nitrogen. It is only synthesized in the presence of these substrates. In nature it is regulated by sRNAs. In this biobrick we altered the ribosome binding site for ChiP regulator – ChiX (AAGAGG-->AGGAGA). It was done in order to show that the regulatory system depends on the specific base pairing between ChiP and its regulator. The promoter and reporter have not been altered compared to K564012.
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ChiP encodes enterobacterial chitoporin required for uptake of chitin-derived oligosaccharides. This outer membrane porin is required for Salmonella and E.coli growth on chitooligosaccharides as a sole source of both carbon and nitrogen. It is only synthesized in the presence of these substrates. In nature it is regulated by sRNAs. In this biobrick we altered the ribosome binding site for ChiP regulator – ChiX (AAGAGG-->AGGAGA). It was done in order to show that the regulatory system depends on the specific base pairing between ChiP and its regulator. The promoter and reporter have not been altered compared to [https://parts.igem.org/wiki/index.php?title=Part:BBa_K564012 K564012].
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This was verified by β-gal assays as shown in the table below. The measurements are in Miller Units
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{| class="wikitable"
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|-
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! Strain
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! [http://ecoliwiki.net/colipedia/index.php/NM522 NM522]
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! [http://2011.igem.org/Team:DTU-Denmark/Project_testing_sRNA#Strain_construction IG302]
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|-
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| Genotype
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| chiX +
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| chiX -
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|-
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| [https://parts.igem.org/wiki/index.php?title=Part:BBa_K564012 '''PchiP(original):lacZ, BBa_K564012''']
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| 467
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| 4781
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|-
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| [https://parts.igem.org/wiki/index.php?title=Part:BBa_K564013 '''PchiP(mutated):lacZ, BBa_K564013''']
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| 2353
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| 2403
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|}
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This system will not work in a strain deficient in hfq.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 01:52, 22 September 2011

Upstream mutated chitoporin part fused with lacZ

ChiP encodes enterobacterial chitoporin required for uptake of chitin-derived oligosaccharides. This outer membrane porin is required for Salmonella and E.coli growth on chitooligosaccharides as a sole source of both carbon and nitrogen. It is only synthesized in the presence of these substrates. In nature it is regulated by sRNAs. In this biobrick we altered the ribosome binding site for ChiP regulator – ChiX (AAGAGG-->AGGAGA). It was done in order to show that the regulatory system depends on the specific base pairing between ChiP and its regulator. The promoter and reporter have not been altered compared to K564012.

This was verified by β-gal assays as shown in the table below. The measurements are in Miller Units

Strain [http://ecoliwiki.net/colipedia/index.php/NM522 NM522] [http://2011.igem.org/Team:DTU-Denmark/Project_testing_sRNA#Strain_construction IG302]
Genotype chiX + chiX -
PchiP(original):lacZ, BBa_K564012 467 4781
PchiP(mutated):lacZ, BBa_K564013 2353 2403

This system will not work in a strain deficient in hfq.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]