Difference between revisions of "Part:BBa K564013"

Revision as of 01:51, 22 September 2011

Upstream mutated chitoporin part fused with lacZ

ChiP encodes enterobacterial chitoporin required for uptake of chitin-derived oligosaccharides. This outer membrane porin is required for Salmonella and E.coli growth on chitooligosaccharides as a sole source of both carbon and nitrogen. It is only synthesized in the presence of these substrates. In nature it is regulated by sRNAs. In this biobrick we altered the ribosome binding site for ChiP regulator – ChiX (AAGAGG-->AGGAGA). It was done in order to show that the regulatory system depends on the specific base pairing between ChiP and its regulator. The promoter and reporter have not been altered compared to K564012.

This was verified by β-gal assays as shown in the table below. The measurements are in Miller Units

This system will not work in a strain deficient in hfq.

Sequence and Features