Difference between revisions of "Part:BBa K613014:Design"

 
(→‎Design Notes)
 
(One intermediate revision by the same user not shown)
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K613014 short</partinfo>
 
<partinfo>BBa_K613014 short</partinfo>
Line 7: Line 6:
  
 
===Design Notes===
 
===Design Notes===
−
The mutation was introduced by site-directed mutagenesis.
+
The mutation was introduced by PCR-induced mutagenesis, following data about the V36F mutation in based in [http://www.sciencedirect.com/science/article/pii/S0378111907004623 Krueger et al, 2007].
−
 
+
−
 
+
  
 
===Source===
 
===Source===

Latest revision as of 01:24, 22 September 2011

TetR V36FW43S mutant


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The mutation was introduced by PCR-induced mutagenesis, following data about the V36F mutation in based in [http://www.sciencedirect.com/science/article/pii/S0378111907004623 Krueger et al, 2007].

Source

The TetR gene was PCR-amplified from the Repressilator plasmid. We then introduced the V36FW43S mutation by site-directed mutagenesis. The V36F mutation had already been described by Krueger et al (2007).

References