Difference between revisions of "Part:BBa K613020:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | The mutation was introduced by site-directed mutagenesis | + | The mutation was introduced by site-directed mutagenesis, based on data from [http://www.sciencedirect.com/science/article/pii/S0022283697915400 Helbl and Hillen, 1998]. |
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===Source=== | ===Source=== | ||
Revision as of 01:04, 22 September 2011
TetR P39Q Y42M L197S mutant
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The mutation was introduced by site-directed mutagenesis, based on data from [http://www.sciencedirect.com/science/article/pii/S0022283697915400 Helbl and Hillen, 1998].
Source
The TetR gene was PCR-amplified from the Repressilator plasmid. We then introduced the V36F mutation by site-directed mutagenesis. Parts of this mutation had already been described by Helbl and Hillen (1998).