Difference between revisions of "Part:BBa K613020:Design"
(→Design Notes) |
|||
Line 1: | Line 1: | ||
− | |||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K613020 short</partinfo> | <partinfo>BBa_K613020 short</partinfo> | ||
Line 7: | Line 6: | ||
===Design Notes=== | ===Design Notes=== | ||
− | The mutation was introduced by site-directed mutagenesis | + | The mutation was introduced by site-directed mutagenesis, based on data from [http://www.sciencedirect.com/science/article/pii/S0022283697915400 Helbl and Hillen, 1998]. |
− | + | ||
− | + | ||
===Source=== | ===Source=== |
Revision as of 01:04, 22 September 2011
TetR P39Q Y42M L197S mutant
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The mutation was introduced by site-directed mutagenesis, based on data from [http://www.sciencedirect.com/science/article/pii/S0022283697915400 Helbl and Hillen, 1998].
Source
The TetR gene was PCR-amplified from the Repressilator plasmid. We then introduced the V36F mutation by site-directed mutagenesis. Parts of this mutation had already been described by Helbl and Hillen (1998).