Difference between revisions of "Part:BBa K613014:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | The mutation was introduced by site-directed mutagenesis. | + | The mutation was introduced by site-directed mutagenesis, following data about the V36F mutation in based in [http://www.sciencedirect.com/science/article/pii/S0378111907004623 Krueger et al, 2007]. |
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===Source=== | ===Source=== |
Revision as of 00:54, 22 September 2011
TetR V36FW43S mutant
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The mutation was introduced by site-directed mutagenesis, following data about the V36F mutation in based in [http://www.sciencedirect.com/science/article/pii/S0378111907004623 Krueger et al, 2007].
Source
The TetR gene was PCR-amplified from the Repressilator plasmid. We then introduced the V36FW43S mutation by site-directed mutagenesis. The V36F mutation had already been described by Krueger et al (2007).