Difference between revisions of "Part:BBa K678002"

Revision as of 00:04, 22 September 2011

eGFP-PTS1 for mammalian cells

Vector expressing eGFP targeted to the peroxisomes with the targeting sequence S-K-L often referred to as peroxisomal targeting sequence 1 (PTS1) from the cytomegalovirus (CMV) promoter with the BGH polyA tail and with a hygromycin resistance cassette.

Usage and Biology

We used this device to target enhanced GFP to the peroxisomes of the mammalian cell line U-2 OS (See picture below).

This picture shows the mammalian cell line U-2 OS transfected with device BBa_K6788002 on a plasmid and a plasmid expressing Cyan fluorescent protein.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 2233
Illegal SpeI site found at 49
Illegal PstI site found at 2323
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 2233
Illegal SpeI site found at 49
Illegal PstI site found at 2323
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 2233
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 2233
Illegal SpeI site found at 49
Illegal PstI site found at 2323
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 2233
Illegal SpeI site found at 49
Illegal PstI site found at 2323
Illegal NgoMIV site found at 3088
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K678002 short</partinfo>
-Vector expressing eGFP from the CMV promoter with the BGH polyA tail and with a hygromycin resistance cassette targeted to the peroxisomes with the targeting sequence S-K-L often referred to as peroxisomal targeting sequence 1 (PTS1)
+Vector expressing eGFP targeted to the peroxisomes with the targeting sequence S-K-L often referred to as peroxisomal targeting sequence 1 (PTS1) from the cytomegalovirus (CMV) promoter with the BGH polyA tail and with a hygromycin resistance cassette.
 ===Usage and Biology===