Difference between revisions of "Part:BBa K607031"
 
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<partinfo>BBa_K607031 short</partinfo>
 
<partinfo>BBa_K607031 short</partinfo>
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This part is a member of [https://parts.igem.org/LasR_autoinducing_loop_family LasR autoinducing loop family].
  
 
LasR protein without degradation tag under transcriptional control of PlasB promoter and reverse antisense pBad/araC promoter, the RBS has a strength of 0.07 (obtained by Jason Kelly and Robbie Bryant during the summer of 2004 using a fluorescent reporter). This construct represents (in presence of N-(3-oxododecanoyl)homoserine lactone in the media) an auto-inducing loop in which the PlasB promoter drives expression of its own inducer LasR. The running loop can be turned off through activation of the reverse antisense PBad promoter by arabinose.  
 
LasR protein without degradation tag under transcriptional control of PlasB promoter and reverse antisense pBad/araC promoter, the RBS has a strength of 0.07 (obtained by Jason Kelly and Robbie Bryant during the summer of 2004 using a fluorescent reporter). This construct represents (in presence of N-(3-oxododecanoyl)homoserine lactone in the media) an auto-inducing loop in which the PlasB promoter drives expression of its own inducer LasR. The running loop can be turned off through activation of the reverse antisense PBad promoter by arabinose.  
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[[Image:LasR9.png|Caption test]]
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[[Image:lciaulegend.png|frameless]]
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'''Figure 1.''' This autoinducing loop does not exhibit heterogenity when induced. One populations not producing RFP is visible. That means that the loop is not induced.
  
 
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Latest revision as of 23:45, 21 September 2011

PlasB_LasR_antipBad/araC with 0.07RBS

This part is a member of LasR autoinducing loop family.

LasR protein without degradation tag under transcriptional control of PlasB promoter and reverse antisense pBad/araC promoter, the RBS has a strength of 0.07 (obtained by Jason Kelly and Robbie Bryant during the summer of 2004 using a fluorescent reporter). This construct represents (in presence of N-(3-oxododecanoyl)homoserine lactone in the media) an auto-inducing loop in which the PlasB promoter drives expression of its own inducer LasR. The running loop can be turned off through activation of the reverse antisense PBad promoter by arabinose.

Caption test Lciaulegend.png

Figure 1. This autoinducing loop does not exhibit heterogenity when induced. One populations not producing RFP is visible. That means that the loop is not induced.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 195
    Illegal NheI site found at 1054
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1115
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 644
    Illegal AgeI site found at 841
    Illegal AgeI site found at 1280
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1297