Difference between revisions of "Part:BBa K638404"
Line 3: Line 3:
  
 
This is our [[Part:BBa_K638101 | reflectin A1]] biobrick with an N-terminal 6-His tag, with the ribosome binding site [[Part:BBa_B0034 | B0034]].
 
This is our [[Part:BBa_K638101 | reflectin A1]] biobrick with an N-terminal 6-His tag, with the ribosome binding site [[Part:BBa_B0034 | B0034]].
 +
 +
The coding region is for the [http://www.ncbi.nlm.nih.gov/nuccore/FJ824804.1 Reflectin A1 protein], originally found in the mantle of squid ''Loligo pealeii''. It has been codon optimised for ''E. coli''.
 +
[http://2011.igem.org/Team:Cambridge Cambridge 2011] overexpressed it for purification using the his-tagged generator [[Part:BBa_K638202 | BBa_K638202 ]], and used it to produce brightly coloured responsive thin films. See our [http://2011.igem.org/Team:Cambridge/Project/Microscopy#/Media#Dynamic_Iridescence reflectin thin films] in action or read more about [http://2011.igem.org/Team:Cambridge/Project/Background the background to this protein].
 +
 +
===Usage and Biology===
 +
In the squid this protein is believed to self assemble with membranes to form iridophore platelets - stacks of protein ultrastructure evenly spaced to form a Bragg stack.  This creates living structural colour from thin film interference effects.  In ''L. pealeii'' reflectins are phosphorylated, with [http://www.ncbi.nlm.nih.gov/pubmed/19776150 changes in phosphorylation status correlating with changes in tissue colour].  Read more about [http://2011.igem.org/Team:Cambridge/Project/Background the background to this protein].
 +
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 23:34, 21 September 2011

polyHis-ReflectinA1

This is our reflectin A1 biobrick with an N-terminal 6-His tag, with the ribosome binding site B0034.

The coding region is for the [http://www.ncbi.nlm.nih.gov/nuccore/FJ824804.1 Reflectin A1 protein], originally found in the mantle of squid Loligo pealeii. It has been codon optimised for E. coli. [http://2011.igem.org/Team:Cambridge Cambridge 2011] overexpressed it for purification using the his-tagged generator BBa_K638202 , and used it to produce brightly coloured responsive thin films. See our [http://2011.igem.org/Team:Cambridge/Project/Microscopy#/Media#Dynamic_Iridescence reflectin thin films] in action or read more about [http://2011.igem.org/Team:Cambridge/Project/Background the background to this protein].

Usage and Biology

In the squid this protein is believed to self assemble with membranes to form iridophore platelets - stacks of protein ultrastructure evenly spaced to form a Bragg stack. This creates living structural colour from thin film interference effects. In L. pealeii reflectins are phosphorylated, with [http://www.ncbi.nlm.nih.gov/pubmed/19776150 changes in phosphorylation status correlating with changes in tissue colour]. Read more about [http://2011.igem.org/Team:Cambridge/Project/Background the background to this protein].


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 43
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]