Difference between revisions of "Part:BBa K638103"

 
 
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<partinfo>BBa_K638103 short</partinfo>
 
<partinfo>BBa_K638103 short</partinfo>
  
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Arabinose inducible Reflectin 1B generator - best used with Bw27783 - an Ecoli strain with constitutive expression of an arabinose transporter.
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Produces [[Part:BBa_K638003 | Reflectin 1B]] protein on addition of arabinose.
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The reflectin gene is under the control of the pBAD promoter [[Part:BBa_I0500 | BBa_I0500]], which is tightly controlled by two factors:
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*'''L-arabinose''' monosaccharide taken up by the cell from the medium, which acts as an ''inducer''.
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*'''AraC''' protein included in the I0500 biobrick, which acts an a ''repressor''.
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Therefore, the araC-pBAD system offers regulatable control of gene expression in the presence of the inducer and highly repressed in the absence of the inducer.
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Read more about [http://2011.igem.org/Team:Cambridge/Experiments/Low_Level_Expression the pBAD and arabinose system in E.coli].  
  
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===Usage and Biology===
 
===Usage and Biology===
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Best used in an ''E. coli'' chassis such as strain [http://cgsc.biology.yale.edu/Strain.php?ID=111773 BW27783], with constitutive expression of an Arabinose transporter.
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See our [[Part:BBa_I0500:Experience | experience of pBAD]] for some issues with tuning expression levels.
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====Safety====
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The protein coding sequence for reflectin originally came from cells of an edible squid. There have been no reported safety issues for reflectins, so we do not anticipate the need for extra precautions when using this BioBrick part. See our [http://2011.igem.org/Team:Cambridge/Safety safety page] for more information.
  
 
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Latest revision as of 23:22, 21 September 2011

Arabinose inducible Reflectin 1B generator

Produces Reflectin 1B protein on addition of arabinose.

The reflectin gene is under the control of the pBAD promoter BBa_I0500, which is tightly controlled by two factors:

  • L-arabinose monosaccharide taken up by the cell from the medium, which acts as an inducer.
  • AraC protein included in the I0500 biobrick, which acts an a repressor.

Therefore, the araC-pBAD system offers regulatable control of gene expression in the presence of the inducer and highly repressed in the absence of the inducer. Read more about [http://2011.igem.org/Team:Cambridge/Experiments/Low_Level_Expression the pBAD and arabinose system in E.coli].

Usage and Biology

Best used in an E. coli chassis such as strain [http://cgsc.biology.yale.edu/Strain.php?ID=111773 BW27783], with constitutive expression of an Arabinose transporter. See our experience of pBAD for some issues with tuning expression levels.

Safety

The protein coding sequence for reflectin originally came from cells of an edible squid. There have been no reported safety issues for reflectins, so we do not anticipate the need for extra precautions when using this BioBrick part. See our [http://2011.igem.org/Team:Cambridge/Safety safety page] for more information.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 1260
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961