Difference between revisions of "Part:BBa K638102"
 
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<partinfo>BBa_K638102 short</partinfo>
 
<partinfo>BBa_K638102 short</partinfo>
  
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Arabinose inducible [[Part:BBa_K638002|Reflectin A2]] generator.   
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Produces [[Part:BBa_K638002|Reflectin A2]] protein on addition of arabinose.   
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The reflectin gene is under the control of the pBAD promoter [[Part:BBa_I0500 | BBa_I0500]], which is tightly controlled by two factors:
 +
*'''L-arabinose''' monosaccharide taken up by the cell from the medium, which acts as an ''inducer''.
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*'''AraC''' protein included in the I0500 biobrick, which acts an a ''repressor''.
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Therefore, the araC-pBAD system offers regulatable control of gene expression in the presence of the inducer and highly repressed in the absence of the inducer.
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Read more about [http://2011.igem.org/Team:Cambridge/Experiments/Low_Level_Expression the pBAD and arabinose system in E.coli].
  
 
===Usage and Biology===
 
===Usage and Biology===
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Best used in an ''E. coli'' chassis such as strain Bw27783, with constitutive expression of an Arabinose transporter.
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Best used in an ''E. coli'' chassis such as the strain [http://cgsc.biology.yale.edu/Strain.php?ID=111773 BW27783], with constitutive expression of an Arabinose transporter.
 
See our [[Part:BBa_I0500:Experience | experience of pBAD]] for some issues with tuning expression levels.
 
See our [[Part:BBa_I0500:Experience | experience of pBAD]] for some issues with tuning expression levels.
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====Safety====
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The protein coding sequence for reflectin originally came from cells of an edible squid. There have been no reported safety issues for reflectins, so we do not anticipate the need for extra precautions when using this BioBrick part. See our [http://2011.igem.org/Team:Cambridge/Safety safety page] for more information.
  
 
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Latest revision as of 23:22, 21 September 2011

Arabinose inducible Reflectin A2 generator

Produces Reflectin A2 protein on addition of arabinose.

The reflectin gene is under the control of the pBAD promoter BBa_I0500, which is tightly controlled by two factors:

  • L-arabinose monosaccharide taken up by the cell from the medium, which acts as an inducer.
  • AraC protein included in the I0500 biobrick, which acts an a repressor.

Therefore, the araC-pBAD system offers regulatable control of gene expression in the presence of the inducer and highly repressed in the absence of the inducer. Read more about [http://2011.igem.org/Team:Cambridge/Experiments/Low_Level_Expression the pBAD and arabinose system in E.coli].

Usage and Biology

Best used in an E. coli chassis such as the strain [http://cgsc.biology.yale.edu/Strain.php?ID=111773 BW27783], with constitutive expression of an Arabinose transporter. See our experience of pBAD for some issues with tuning expression levels.

Safety

The protein coding sequence for reflectin originally came from cells of an edible squid. There have been no reported safety issues for reflectins, so we do not anticipate the need for extra precautions when using this BioBrick part. See our [http://2011.igem.org/Team:Cambridge/Safety safety page] for more information.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 1308
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961