Difference between revisions of "Part:BBa K627011"
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<partinfo>BBa_K627011 short</partinfo><br> | <partinfo>BBa_K627011 short</partinfo><br> | ||
− | This BioBrick is a 2 part fusion of arabinose-inducible induction system and the HRV 14_3C protease. The recombinant type 14_3C protease from human rhinovirus (HRV 3C) recognizes the same cleavage site as the native enzyme: | + | |
− | The | + | ====Introduction==== |
− | The arabinose-inducible induction system was amplified via PCR from the pBAD_iGEMexpress vector | + | This BioBrick is a 2 part fusion of arabinose-inducible induction system and the HRV 14_3C protease. |
+ | ====General Information of the single sub parts==== | ||
+ | =====HRV 14_3C===== | ||
+ | The recombinant type 14_3C protease from human rhinovirus (HRV 3C) recognizes the same cleavage site as the native enzyme: LeuGluValLeuPheGln/GlyPro.<br> | ||
+ | The 22 kDa protease got its optimal activity at 4°C but also shows a high cleavage rate at 37°C. The 14_3C works with a catalytic triade, containing the amino acid residues Ser-Asp-His at its active site.<br> | ||
+ | =====Arabinose-inducible induction system===== | ||
+ | The arabinose-inducible induction system was amplified via PCR from the pBAD_iGEMexpress vector. It gots a very low expression rate without induction by arabinose. For the induction concentration between 2 mM up to 50 mM arabinose can be used to get an high expression rate. | ||
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Revision as of 23:06, 21 September 2011
Fusion part of pBAD arabinose-inducible induction system and the HRV 14_3C protease
Introduction
This BioBrick is a 2 part fusion of arabinose-inducible induction system and the HRV 14_3C protease.
General Information of the single sub parts
HRV 14_3C
The recombinant type 14_3C protease from human rhinovirus (HRV 3C) recognizes the same cleavage site as the native enzyme: LeuGluValLeuPheGln/GlyPro.
The 22 kDa protease got its optimal activity at 4°C but also shows a high cleavage rate at 37°C. The 14_3C works with a catalytic triade, containing the amino acid residues Ser-Asp-His at its active site.
Arabinose-inducible induction system
The arabinose-inducible induction system was amplified via PCR from the pBAD_iGEMexpress vector. It gots a very low expression rate without induction by arabinose. For the induction concentration between 2 mM up to 50 mM arabinose can be used to get an high expression rate.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1523
Illegal BglII site found at 1711
Illegal BamHI site found at 1144 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1239
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961