Difference between revisions of "Part:BBa K592004:Design"

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===Design Notes===
 
===Design Notes===
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The sequence information was provided in Moglich and Moffat's article. The physical DNA was synthesized by GenScript. Codon-optimization may enhance its expression.  
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The sequence information was provided in the article by Moglich and Moffat referenced below. The physical DNA was synthesized by GenScript. The sequence uses the wild-type codon usage, no codon optimization has been done.
  
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===Source===
  
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Ytva comes from the genome of ''Bacillus subtilis''.
  
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===Source===
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FixL derives from the genome of ''Bradyrhizobium japonicum''.
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Ytva comes from the genome of Bacillus subtilis.
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FixL derives from the genome of Bradyrhizobium japonicum.  
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The DNA was synthesized by GenScript and been through quality control. Therefore, this construct was never sequenced on its own. However, it has been sequenced after assembling with other components, notably in <partinfo>BBa_S04616</partinfo>.
  
 
===References===
 
===References===

Latest revision as of 22:54, 21 September 2011

YF1 blue-light sensor protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 544
    Illegal NgoMIV site found at 616
    Illegal NgoMIV site found at 706
    Illegal NgoMIV site found at 724
    Illegal AgeI site found at 258
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 157


Design Notes

The sequence information was provided in the article by Moglich and Moffat referenced below. The physical DNA was synthesized by GenScript. The sequence uses the wild-type codon usage, no codon optimization has been done.

Source

Ytva comes from the genome of Bacillus subtilis.

FixL derives from the genome of Bradyrhizobium japonicum.

The DNA was synthesized by GenScript and been through quality control. Therefore, this construct was never sequenced on its own. However, it has been sequenced after assembling with other components, notably in BBa_S04616.

References

Moglich A, Ayers RA and Moffat K. (2009) Design and Signaling Mechanism of Light-Regulated Histidine Kinases. J. Mol. Bio. 385, 5, 1433-1444.