Difference between revisions of "Part:BBa K537010"
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This composite BioBrick begins with a strong, constitutively active promoter of E.coli (BBa_J23119) followed by a theophylline riboswitch (type2) (Lynch and Gallivan NAR 2009) which is fused to the Venus fluorescent reporter protein without a stop codon in between. The theophylline riboswitch2-venus fusion was constructed via 2 rounds of PCR. This part ends with a standard double terminator transcriptional terminator for E.coli (BBa_B0015). | This composite BioBrick begins with a strong, constitutively active promoter of E.coli (BBa_J23119) followed by a theophylline riboswitch (type2) (Lynch and Gallivan NAR 2009) which is fused to the Venus fluorescent reporter protein without a stop codon in between. The theophylline riboswitch2-venus fusion was constructed via 2 rounds of PCR. This part ends with a standard double terminator transcriptional terminator for E.coli (BBa_B0015). | ||
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| + | <html> | ||
| + | <center><img src="https://static.igem.org/mediawiki/2011/4/47/Datasheet2a.jpg" width="910" vspace="15"></center> | ||
| + | <p>For the pdf version of this datasheet, click <a href="https://static.igem.org/mediawiki/parts/f/f5/Datasheet2.pdf">here</a></p> | ||
| + | </html> | ||
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Revision as of 22:39, 21 September 2011
Promoter-Theophylline riboswitch 2-Venus-Double terminator
This composite BioBrick begins with a strong, constitutively active promoter of E.coli (BBa_J23119) followed by a theophylline riboswitch (type2) (Lynch and Gallivan NAR 2009) which is fused to the Venus fluorescent reporter protein without a stop codon in between. The theophylline riboswitch2-venus fusion was constructed via 2 rounds of PCR. This part ends with a standard double terminator transcriptional terminator for E.coli (BBa_B0015).
For the pdf version of this datasheet, click here
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 730
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]