Difference between revisions of "Part:BBa K528015"
 
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<partinfo>BBa_K528015 short</partinfo>
 
<partinfo>BBa_K528015 short</partinfo>
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45k is strength in Voight's virtual units.
 
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===Construct design===
 
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To make truly standardize strength of RBS parts we propose a brand new part in synthetic biology - we call expression adapters. They are short sequences consisting of RBS, 5 bp spacer and a short (10 amino acid long) beginning of a protein.
 
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For more info please look at Team Warsaw 2011 http://2011.igem.org/Team:Warsaw/ExpressionAdaptors/Solution
 
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===Authors===
 
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Clonning:
 
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*Paweł Urbański
 
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*Elżbieta Jankowska
 
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*Krzysztof Szczepaniak
 
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*Dorota Kaja Sabat
 
 
   
 
   
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The lab work was supervised by:
 
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*Michał Lower
 
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*Ania Olchowik
 
  
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{{ExpressionAdaptor|strength=64,29%}}
 
   
 
   
  

Latest revision as of 22:34, 21 September 2011

Expression Adapter s00_45k


This part encodes expression adaptor designed to have strength 64,29% relative to B0034 and uniform expression level regardless of attached protein.

Authors

Clonning:

  • Paweł Urbański
  • Elżbieta Jankowska
  • Krzysztof Szczepaniak
  • Dorota Kaja Sabat

The lab work was supervised by:

  • Michał Lower
  • Ania Olchowik


Construct design

The part is in RFC25 standard. It consists of BBa_J23100 promoter followed by BBa_B0034 RBS, 5 nucleotide spacer and short peptide.

Expression_Adapter.png

Spacer and peptide were designed using in silico evolution to provide uniform expression level regardless of following protein coding sequence.


Summary of design algorithm:


Genetic.png

Measurements

Using RBSCalculator program we have determined that this part expression strength is 64,29% relative to BBa_B0034

All our measurement results are summarized in the table below:


Part NameDescriptionExpression level in thousands of absolute RBSDesigner unitsExpression level as %B0034+YFP
BBa_K528015Expression Adapters s00_45k4564,29%
BBa_K528016Expression Adapters s01_24k2434,29%
BBa_K528017expression adapters s02_20k2028,57%
BBa_K528018Expression Adapters s06_16k1622,86%
BBa_K528019Expression Adapters s05_12k1217,14%
BBa_K528020expression adapters s03_65k6592,86%
BBa_K528021BBa_K528018+YFP measurement device1622,86%
referenceBBa_J23102+B0034+YFP (100% reference construct)70100,00%


For more info please look at Team Warsaw 2011 [http://2011.igem.org/Team:Warsaw/ExpressionAdaptors wiki page].


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 8
    Illegal NheI site found at 31
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]