Difference between revisions of "Part:BBa K525221:Design"
(→‎Source)
 
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===Source===
 
===Source===
−
* PCR on the Corynebacterium halotolerans DNA cloned into BioBrick Vectors
+
* PCR on the Corynebacterium halotolerans ([http://www.dsmz.de/microorganisms/html/strains/strain.dsm044683.html DSM No. 44683)] DNA cloned into BioBrick Vectors
 
** Primers with BioBrick prefix, pT7-Promoter and RBS in the fwd primer. Suffix in the rev primer.  
 
** Primers with BioBrick prefix, pT7-Promoter and RBS in the fwd primer. Suffix in the rev primer.  
 
*** fwd: GAATTCGCGGCCGCTTCTAGAGTAATACGACTCACTATAGGGAAAGAGGAGAAAATGATCAAGAACCGCATCCGCAC
 
*** fwd: GAATTCGCGGCCGCTTCTAGAGTAATACGACTCACTATAGGGAAAGAGGAGAAAATGATCAAGAACCGCATCCGCAC

Latest revision as of 22:23, 21 September 2011

S-layer cspB from Corynebacterium halotolerans with TAT-Sequence and lipid anchor, PT7 and RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1191
    Illegal XhoI site found at 647
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 314
    Illegal NgoMIV site found at 1403
    Illegal NgoMIV site found at 1514
    Illegal AgeI site found at 89
    Illegal AgeI site found at 305
    Illegal AgeI site found at 546
    Illegal AgeI site found at 593
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 995
    Illegal BsaI.rc site found at 302
    Illegal BsaI.rc site found at 680
    Illegal BsaI.rc site found at 1082


Design Notes

This is the complete cspB S-layer sequence with TAT sequence and with lipid anchor.


Source

  • PCR on the Corynebacterium halotolerans ([http://www.dsmz.de/microorganisms/html/strains/strain.dsm044683.html DSM No. 44683)] DNA cloned into BioBrick Vectors
    • Primers with BioBrick prefix, pT7-Promoter and RBS in the fwd primer. Suffix in the rev primer.
      • fwd: GAATTCGCGGCCGCTTCTAGAGTAATACGACTCACTATAGGGAAAGAGGAGAAAATGATCAAGAACCGCATCCGCAC
      • rev: CTGCAGCGGCCGCTACTAGTATTAGCGGTTCAGGTTCAGGAAG

References