Difference between revisions of "Part:BBa K676001"
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From Pato(1990) it was found out that the wild type phage can carry out replication effectively compare to the mutant which lacks the GBS after the heat induction of the lysogens . | From Pato(1990) it was found out that the wild type phage can carry out replication effectively compare to the mutant which lacks the GBS after the heat induction of the lysogens . | ||
| + | By introducing the Mu GBS into the target plasmids, we achieved a higher level of supercoiling as well as obtaining a more uniformly supercoiled products . Highly supercoiled plasmids are able to resist shear stress better and this property plays an important role in large scale plasmid DNA manufacturing as well as gene therapy delivery. | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Revision as of 22:16, 21 September 2011
Gyrase Binding Site from Mu Bacteriophage
The strong gyrase site clones from Mu bacteriophage.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 191
- 1000COMPATIBLE WITH RFC[1000]