Difference between revisions of "Part:BBa K628006:Design"
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<partinfo>BBa_K628006 short</partinfo> | <partinfo>BBa_K628006 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
| − | + | Protegrin-1 coding region sequenced by Gene Art assembled by standard biobrick assembly. This has been inserted into the standard iGEM 2011 backbone pSB1C3 which has chloramphenicol resistance. | |
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===Source=== | ===Source=== | ||
| − | + | The part has been optimised for e coli and designed with a pBad strong promoter to allow lyses to be controlled by varying the concentration of arabinose. This could be altered in future applications and a more specific promoter could be used to allow regulation of the switch to be tightly linked to conditions in the proposed environment. | |
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===References=== | ===References=== | ||
Latest revision as of 21:43, 21 September 2011
Protegrin-1 Kill Switch
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 125
Illegal NotI site found at 164 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 65
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Protegrin-1 coding region sequenced by Gene Art assembled by standard biobrick assembly. This has been inserted into the standard iGEM 2011 backbone pSB1C3 which has chloramphenicol resistance.
Source
The part has been optimised for e coli and designed with a pBad strong promoter to allow lyses to be controlled by varying the concentration of arabinose. This could be altered in future applications and a more specific promoter could be used to allow regulation of the switch to be tightly linked to conditions in the proposed environment.