Difference between revisions of "Part:BBa K607018"

Latest revision as of 21:40, 21 September 2011

Prm_cI-DAS_antiPlasB with 1.0RBS

This part is a member of Lambda cI autoinducing loop family.

cI with moderately fast tag under transcriptional control of Prm promoter and reverse antisense lasB promoter, the RBS has a strength of 1.0 (obtained by Jason Kelly and Robbie Bryant during the summer of 2004 using a fluorescent reporter). This construct represents an auto-inducing loop in which the Prm promoter drives expression of its own inducer cI. The running loop can be turned off by activating the reverse antisense lasB promoter (through LasR protein in the presence of Pseudomonas autoinducer (PAI))

Figure 1. This autoinducing loop does exhibit heterogenity when non-induced. Two populations are visible - a population that is not producing GFP and a population that is producing GFP is observed. After 4 hours of incubation approximately 36,45% of the visible cells are fluorescent while 61,88% are not fluorescent. That means that the loop is induced in some of the cells through a leaky promoter. Changing the promoter to a less leaky version might make it a good candidate for an inducible bistable switch.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1100
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K607018 short</partinfo>
+This part is a member of [https://parts.igem.org/Lambda_cI_autoinducing_loop_family Lambda cI autoinducing loop family].
 cI with moderately fast tag under transcriptional control of Prm promoter and reverse antisense lasB promoter, the RBS has a strength of 1.0 (obtained by Jason Kelly and Robbie Bryant during the summer of 2004 using a fluorescent reporter). This construct represents an auto-inducing loop in which the Prm promoter drives expression of its own inducer cI. The running loop can be turned off by activating the reverse antisense lasB promoter (through LasR protein in the presence of Pseudomonas autoinducer (PAI))
+[[Image:lciau7.png|Caption test]]
+[[Image:lciaulegend.png|frameless]]
+'''Figure 1.''' This autoinducing loop does exhibit heterogenity when non-induced. Two populations are visible - a population that is not producing GFP and a population that is producing GFP is observed. After 4 hours of incubation approximately 36,45% of the visible cells are fluorescent while 61,88% are not fluorescent. That means that the loop is induced in some of the cells through a leaky promoter. Changing the promoter to a less leaky version might make it a good candidate for an inducible bistable switch.
 <!-- Add more about the biology of this part here