Difference between revisions of "Part:BBa K525730:Design"
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===Source=== | ===Source=== | ||
| − | Escherichia coli | + | *PCR on the '' Escherichia coli'' TOP 10 DNA cloned into BioBrick Vectors |
| + | *Primers with BioBrick prefix, T7-promoter and RBS in the fwd primer. Freiburg Suffix in the rev primer. | ||
| + | **fwd: 5'-acgtgaattcgcggccgcttctagagtaatacgactcactatagggaaagaggagaaaaatggaatcaatcgaacaac-3' | ||
| + | **rev: 5'-acgtctgcagcggccgctactagtattaaccggtgctacccagcaaacgc-3' | ||
===References=== | ===References=== | ||
Revision as of 21:40, 21 September 2011
DNA ligase from Escherichia coli (LigA) with PT7 and RBS
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1449
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 2047
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 1663
Design Notes
- This is the complete sequence of NAD+ -dependent DNA ligase with T7-promoter and RBS.
- It is designed in RFC 25 -suffix standard
Source
- PCR on the Escherichia coli TOP 10 DNA cloned into BioBrick Vectors
- Primers with BioBrick prefix, T7-promoter and RBS in the fwd primer. Freiburg Suffix in the rev primer.
- fwd: 5'-acgtgaattcgcggccgcttctagagtaatacgactcactatagggaaagaggagaaaaatggaatcaatcgaacaac-3'
- rev: 5'-acgtctgcagcggccgctactagtattaaccggtgctacccagcaaacgc-3'