Difference between revisions of "Part:BBa K613022"

Latest revision as of 21:29, 21 September 2011

TetR E37A P39K mutant

This is a TetR mutant that carries the E37A P39K double mutation. Part of the EPFL2011 muTetR collection.

It has an altered tet operator sequence recognition compared to the wild-type tetR variant. And prefers the tetO-4C5G operator.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Designed sequence (2 mutations in yellow):

MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVAQKTLYWHVKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCA
LLSHRDGAKVHLGTRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTTDSMPPLLRQAIELFD

HQGAEPAFLFGLELIICGLEKQLKCESGS*

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K613022 short</partinfo>
-This is a TetR mutant with the EA37PK39 double mutation
+This is a TetR mutant that carries the E37A P39K double mutation.
+Part of the <html> <a href="http://2011.igem.org/Team:EPF-Lausanne/Our_Project/TetR_mutants/muTetRs">EPFL2011 muTetR collection. </a> </html>
+It has an altered tet operator sequence recognition compared to the wild-type tetR variant. And prefers the tetO-4C5G operator.
 <!-- Add more about the biology of this part here
@@ Line 10: / Line 16: @@
 <span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_K613022 SequenceAndFeatures</partinfo>
-<p class=MsoNormal><span class=SpellE><span class=GramE><b style='mso-bidi-font-weight:
-normal'><span lang=EN-GB style='font-size:14.0pt;mso-bidi-font-size:10.0pt;
-font-family:"Courier New";mso-bidi-font-family:"Times New Roman";mso-ansi-language:
-EN-GB'>MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGV<span style='background:yellow'>A</span>QPTLYWHVKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS*</span></b></span></span><b
-style='mso-bidi-font-weight:normal'><span lang=EN-GB style='font-size:14.0pt;
-mso-bidi-font-size:10.0pt;font-family:"Courier New";mso-bidi-font-family:"Times New Roman";
-mso-ansi-language:EN-GB'></span></b></p>
+Designed sequence (2 mutations in yellow):
+'''MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGV'''<span style='background:yellow'>'''A'''</span>'''Q'''<span style='background:yellow'>'''K'''</span>'''TLYWHVKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCA<p>LLSHRDGAKVHLGTRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTTDSMPPLLRQAIELFD</p><p>HQGAEPAFLFGLELIICGLEKQLKCESGS*'''</p>
 <!-- Uncomment this to enable Functional Parameter display