Difference between revisions of "Part:BBa K628006"
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<span class='h3bb'>Sequence and Features</span>
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===Sequence and Features===
 
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<partinfo>BBa_K628006 SequenceAndFeatures</partinfo>
  

Revision as of 21:24, 21 September 2011

Protegrin-1 Kill Switch

TBA


Usage and Biology

Protegrin-1 coding region under the influence of a pBad-strong promotor. Protegrin-1 is an '18-residue beta-sheet peptide isolated from porcine leukocytes with antimicrobial activity against a broad range of microorganisms.' (Steinberg et al 1997) It has its effect by pore membrane disruption (Lam et al 2006) and possibly also by effects such as activation of membrane-damaging proteases (Bierbaum 1985) and has anti-microbial activity against E Coli (Aumelas 2004).

The presence of the pBad promoter allows the expression of Protegrin-1 to be controlled by differing concentrations of arbinose.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Unknown
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    INCOMPATIBLE WITH RFC[23]
    Unknown
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



References

Steinberg et al (1997) 'Protegrin-1: a broad-spectrum, rapidly microbicidal peptide with in vivo activity Antimicrobial Agents and Chemotherapy', Aug 1997, 1738-1742, Vol 41, No. 8

Lam et al (2006) 'Mechanism of Supported Membrane Disruption by Antimicrobial Peptide Protegrin-1' J. Phys. Chem. B, 2006, 110 (42), pp 21282–21286

Bierbaum, G. & Sahl, H.-G (1985) 'Induction of autolysis of Staphylocci by the basic peptide antibiotics pep5 and nisin and their influence on the activity of autolytic enzymes.' Arch. Microbiol. 141, 249 ± 254

Aumelas et al. (2004) 'Synthesis and Solution Structure of the Antimicrobial Peptide Protegrin-1' European Journal of Biochemistry Volume 237, Issue 3, pages 575–583, May 1996