Difference between revisions of "Part:BBa K322127:Experience"
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* as a template for isolation of the red light sensor using PCR. This resulted in the generation of part BBa_K568000, which is the red light sensor without a reporter construct. Therefore, Part BBa_K568000 is an improvement of BBa_K322127, because it improves the ease-of-use. The red light sensor can now be combined with any reporter construct. | * as a template for isolation of the red light sensor using PCR. This resulted in the generation of part BBa_K568000, which is the red light sensor without a reporter construct. Therefore, Part BBa_K568000 is an improvement of BBa_K322127, because it improves the ease-of-use. The red light sensor can now be combined with any reporter construct. | ||
| − | * In testing/characterizing part BBa_K568000. To characterize BBa_K568000 and to examine BBa_K322127 a Miller Assay was conducted after transformation of the parts into ''E. coli'' CP919. A cph8 part (kindly provided by team Uppsala-Sweden) was used as a negative control. All cells were incubated under three conditions: Irradiation with light of | + | * In testing/characterizing part BBa_K568000. To characterize BBa_K568000 and to examine BBa_K322127 a Miller Assay was conducted after transformation of the parts into ''E. coli'' CP919. A cph8 part (kindly provided by team Uppsala-Sweden) was used as a negative control. All cells were incubated under three conditions: Irradiation with light of 626 nm ("shut down wavelength"), irradiation with light of 710 nm ("induction wavelength") and in the dark. The measuring was conducted over 210 minutes and then aborted, because no difference between the samples was visible. This means that parts BBa_K322127 and BBa_K568000 did not work as expected in our set-up. Visit TU Munich 2011 <html><a href="http://2011.igem.org/Team:TU_Munich/lab/notebook#Miller_Assay_for_testing_red_light_sensors">Labbook</a></html> (section "Cloning III + Results", date 20-09-2011) for detailed information. |
===User Reviews=== | ===User Reviews=== | ||
Revision as of 20:41, 21 September 2011
This experience page is provided so that any user may enter their experience using this part.
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how you used this part and how it worked out.
Applications of BBa_K322127
This part was used in the project of the TU Munich 2011 in two ways:
- as a template for isolation of the red light sensor using PCR. This resulted in the generation of part BBa_K568000, which is the red light sensor without a reporter construct. Therefore, Part BBa_K568000 is an improvement of BBa_K322127, because it improves the ease-of-use. The red light sensor can now be combined with any reporter construct.
- In testing/characterizing part BBa_K568000. To characterize BBa_K568000 and to examine BBa_K322127 a Miller Assay was conducted after transformation of the parts into E. coli CP919. A cph8 part (kindly provided by team Uppsala-Sweden) was used as a negative control. All cells were incubated under three conditions: Irradiation with light of 626 nm ("shut down wavelength"), irradiation with light of 710 nm ("induction wavelength") and in the dark. The measuring was conducted over 210 minutes and then aborted, because no difference between the samples was visible. This means that parts BBa_K322127 and BBa_K568000 did not work as expected in our set-up. Visit TU Munich 2011 Labbook (section "Cloning III + Results", date 20-09-2011) for detailed information.
User Reviews
UNIQb466d1405d53e0db-partinfo-00000001-QINU UNIQb466d1405d53e0db-partinfo-00000002-QINU