Difference between revisions of "Part:BBa K608351:Experience"

Revision as of 20:28, 21 September 2011

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K608351

We managed to perform a last-minute, "blind" -ie not knowing if the sequence of the insert was correct-, and preliminary OD-measurement testing of the part in conjuction with (BBa_K608352), as part of our temperature sensitive phage lysis cassette.

We later verified the part sequence. Please refer to our Project Results Page (http://2011.igem.org/Team:Freiburg/Results#Lysis_cassette) for more Info.

The OD values of the measurement are shown in the chart (please do bear in mind that this was a measurement done under deadline-stress, without the possibility to plan and perform something more concrete before the Wiki-Freeze)

LB with Cm (Chloramphenicol) was used as a blank before every (hourly) measurement

Nr1: Insert is only this part ((BBa_K608351))
Nr2: Insert is only (BBa_K608352)
Nr3: Both parts were correctly assembled (1:(BBa_K608351) + 2:(BBa_K608352))

Therefore (BBa_K608351) seems to be functioning, though not tightly. More tests are planned if time allows (ie wiki-unfreeze ;) ) One sure thing is that the correct testing would be done with GFP or RFP instead of (BBa_K608352), and also at different temperatures (probably a 25°C to 42°C gradient).

User Reviews

UNIQ80fc1c076250ff3d-partinfo-00000000-QINU UNIQ80fc1c076250ff3d-partinfo-00000001-QINU

@@ Line 4: / Line 4: @@
 ===Applications of BBa_K608351===
-We managed to perform a last-minute, "blind" -ie not knowing if the sequence of the cloning was correct-, and preliminary OD-measurement testing of the part in conjuction with ([https://parts.igem.org/Part:BBa_K608352 BBa_K608352]), as part of our temperature sensitive phage lysis cassette.
+We managed to perform a last-minute, "blind" -ie not knowing if the sequence of the insert was correct-, and preliminary OD-measurement testing of the part in conjuction with ([https://parts.igem.org/Part:BBa_K608352 BBa_K608352]), as part of our temperature sensitive phage lysis cassette.
 <br>
-We later verified the part sequence. Please refer to our Project Description Page (http://2011.igem.org/Team:Freiburg/Description#The_temperature_sensitive_lysis_cassette) for more Info.
+We later verified the part sequence. Please refer to our Project Results Page (http://2011.igem.org/Team:Freiburg/Results#Lysis_cassette) for more Info.
 <br>