Difference between revisions of "Part:BBa K525710:Design"

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===Design Notes===
 
===Design Notes===
  
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This is the complete sequence of NAD<sup>+<sup> -dependent DNA ligase with PT7, RBS and His-tag
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This is the complete sequence of NAD<sup>+</sup> -dependent DNA ligase with PT7, RBS and His-tag
  
 
===Source===
 
===Source===
  
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PCR on the ''Escherichia coli'' TOP 10 DNA cloned into BioBrick Vectors
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*PCR on the ''Escherichia coli'' TOP 10 DNA cloned into BioBrick Vectors
  
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    Primers with BioBrick prefix, pT7-Promoter and RBS in the fwd primer. Suffix in the rev primer.
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*Primers with BioBrick prefix, pT7-Promoter and RBS in the fwd primer. Suffix and His-tag in the rev primer.
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        fwd:  
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**fwd: 5'-acgtgaattcgcggccgcttctagagtaatacgactcactatagggaaagaggagaaaaatggaatcaatcgaacaac-3'
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        rev:
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**rev: 5'-acgtctgcagcggccgctactagtaatgatgatgatgatgatggctacccagcaaacgc-3'
  
 
===References===
 
===References===

Latest revision as of 20:24, 21 September 2011

DNA ligase from Escherichia coli (LigA) with PT7, RBS and His-tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1449
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1663


Design Notes

This is the complete sequence of NAD+ -dependent DNA ligase with PT7, RBS and His-tag

Source

  • PCR on the Escherichia coli TOP 10 DNA cloned into BioBrick Vectors
  • Primers with BioBrick prefix, pT7-Promoter and RBS in the fwd primer. Suffix and His-tag in the rev primer.
    • fwd: 5'-acgtgaattcgcggccgcttctagagtaatacgactcactatagggaaagaggagaaaaatggaatcaatcgaacaac-3'
    • rev: 5'-acgtctgcagcggccgctactagtaatgatgatgatgatgatggctacccagcaaacgc-3'

References