Difference between revisions of "Part:BBa I757010:Experience"
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| + | The lactamases shows great activity at 37°C and 30°C incubation over night on agar plates. The transformed <i>E.coli</i> cells were able to survive with ampicillin concentrations of 400 µg/ml at 37°C and 300 µg/ml at 30°C. | ||
Revision as of 19:52, 21 September 2011
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Applications of BBa_I757010
This beta lactamase was used in combination with the TorA signal sequence (BBa_K208005) as a new protease activity detector device. The BioBrick parts BBa_K627012 and BBa_K627013 consist of the TorA signal sequence followed by a short peptide sequence used as cleavage site for different proteases (TEV (BBa_K627008-BBa_K627010) and 14_3C (BBa_K627011)) and ends with the beta lactamase.
When E.coli XL1 blue where transformed with our construct, the cells were able to survive at ampicillin concentrations of 400 µg/ml, when incubated on agar plates over night at 37°C, or 300 µg/ml ampicillin, when incubated over night at 30 °C.
User Reviews
UNIQ53f60811d5c78a15-partinfo-00000000-QINU UNIQ53f60811d5c78a15-partinfo-00000001-QINU The lactamases shows great activity at 37°C and 30°C incubation over night on agar plates. The transformed E.coli cells were able to survive with ampicillin concentrations of 400 µg/ml at 37°C and 300 µg/ml at 30°C.