Difference between revisions of "Part:BBa K638001"
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This is the coding region for reflectin protein A1 (originally found in the mantle of squid ''Loligo pealeii'') codon optimised for ''E. coli''. | This is the coding region for reflectin protein A1 (originally found in the mantle of squid ''Loligo pealeii'') codon optimised for ''E. coli''. | ||
| − | [http://2011.igem.org/Team:Cambridge Cambridge 2011] overexpressed it for purification using the his-tagged generator [[Part: | + | [http://2011.igem.org/Team:Cambridge Cambridge 2011] overexpressed it for purification using the his-tagged generator [[Part:BBa_K638202 | BBa_K638202 ]]. We also exported it as a [[Part:BBa_K638401 | TorA fusion]]. |
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 19:51, 21 September 2011
Reflectin A1 from Loligo
This is the coding region for reflectin protein A1 (originally found in the mantle of squid Loligo pealeii) codon optimised for E. coli. [http://2011.igem.org/Team:Cambridge Cambridge 2011] overexpressed it for purification using the his-tagged generator BBa_K638202 . We also exported it as a TorA fusion.
Usage and Biology
In the squid this protein is believed to self assemble with membranes to form iridophore platelets - stacks of protein ultrastructure evenly spaced to form a Bragg stack. This creates living structural colour from thin film interference effects. In L. pealeii reflectins are phosphorylated, with [http://www.ncbi.nlm.nih.gov/pubmed/19776150 changes in phosphorylation status correlating with changes in tissue colour]. Read more about [http://2011.igem.org/Team:Cambridge/Project/Background the background to this protein].
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 22
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]