Difference between revisions of "Part:BBa K638001:Experience"

Revision as of 19:46, 21 September 2011

Applications of BBa_K638001

When extracted and purified, this protein can be used to make optical devices including thin films and diffraction gratings as has been demonstated by [http://www.nature.com/nmat/journal/v6/n7/full/nmat1930.html Kramer et al, 2007.] Additionally, Cambridge iGEM Team 2011 further developed the method for making [http://2011.igem.org/Team:Cambridge/Experiments/Thin_Films thin films].

First the protein must be dissolved in an ionic solvent such as [http://en.wikipedia.org/wiki/Hexafluoro-2-propanol HFIP] (hazardous - use in a fume hood) and then can be coated onto a highly clean smooth substrate. Silicon Wafers and glass microscope slides have been shown to work. A razorblade can be used to scrape across a single drop to form a thin film, but more uniform results can be achieved with the use of a [http://2011.igem.org/Team:Cambridge#/Protocols/Flow_coating flow coater] or a [http://2011.igem.org/Team:Cambridge#/Protocols/Spin_Coating spin coater device].

Fig.1 Schematic diagram of the flow-coating technique for casting thin films of reflectin protein onto a silicon-wafer substrate. Image taken from [http://www.nature.com/nmat/journal/v6/n7/full/nmat1930.html Kramer et al, 2007.]
Fig.2 Reflectin films exhibiting uniform thicknesses cast from a 15% w/w (left) and 10% w/w (right) reflectin/HFIP solution. Scale bar: 2 cm. Image taken from [http://www.nature.com/nmat/journal/v6/n7/full/nmat1930.html Kramer et al, 2007.]
Fig.3 One of the first reflectin films prepared by Cambridge iGEM Team 2011.

Compatibility

Has been shown to work with plasmids:

pSB1A3
pSB3K3
pSB1C3

On high copy-plasmids such as pSB1A3, this protein has been shown to go into inclusion bodies.

User Reviews

UNIQ35bdb3017a7e1bcc-partinfo-00000001-QINU UNIQ35bdb3017a7e1bcc-partinfo-00000002-QINU

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-Image:cam_thin_film.png | Fig.1 Schematic diagram of the flow-coating technique for casting thin films of reflectin protein onto a silicon-wafer substrate. ''Image taken from [http://www.nature.com/nmat/journal/v6/n7/full/nmat1930.html Kramer ''et al'', 2007.]'']]
+Image:cam_thin_film.png | Fig.1 Schematic diagram of the flow-coating technique for casting thin films of reflectin protein onto a silicon-wafer substrate. ''Image taken from [http://www.nature.com/nmat/journal/v6/n7/full/nmat1930.html Kramer ''et al'', 2007.]''
-Image:cam_thin_film_I.png | Fig.2 Reflectin films exhibiting uniform thicknesses cast from a 15% w/w (left) and 10%  w/w (right) reflectin/HFIP solution. Scale bar: 2 cm. ''Image taken from [http://www.nature.com/nmat/journal/v6/n7/full/nmat1930.html Kramer ''et al'', 2007.]'' ]]
+Image:cam_thin_film_I.png | Fig.2 Reflectin films exhibiting uniform thicknesses cast from a 15% w/w (left) and 10%  w/w (right) reflectin/HFIP solution. Scale bar: 2 cm. ''Image taken from [http://www.nature.com/nmat/journal/v6/n7/full/nmat1930.html Kramer ''et al'', 2007.]''
+Image:cam_thin_film_II.jpg | Fig.3 One of the first reflectin films prepared by Cambridge iGEM Team 2011.
 </gallery>
 </center>