Difference between revisions of "Part:BBa K607005"

Latest revision as of 19:39, 21 September 2011

Prm_crRNA_LasR-LVA

crRNA coupled LasR with LVA-tag under transcriptional control of Prm promoter.
The crRNA is composed of the sequence of the target gene and an upstream short nucleotide sequence which is complementary to the ribosome binding site (RBS). Repression of gene expression is achieved through sequestration of the RBS by formation of a stem loop structure by the 5'-untranslated region of the crRNA. This prevents binding of the RBS by the 30s ribosomal subunit and subsequent translation.
For more information about the LasR-LVA, see https://parts.igem.org/wiki/index.php?title=Part:BBa_K607002
For more information about the Prm promotor, see: https://parts.igem.org/wiki/index.phptitle=Part:BBa_K607003title=Part:BBa_K607003
References:
Isaacs at al. 2004

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 458
Illegal AgeI site found at 655
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K607005 short</partinfo>
-crRNA coupled LasR with LVA-tag under transcriptional control of Prm promoter
+crRNA coupled LasR with LVA-tag under transcriptional control of Prm promoter.
-The crRNA is composed of the sequence of the target gene and an upstream short nucleotide sequence which is complementary to the ribosome binding site (RBS). Repression of gene expression is achieved through sequestration of the RBS by formation of a stem loop structure by the 5'-untranslated region of the crRNA. This prevents binding of the RBS by the 30s ribosomal subunit and subsequent translation.
+<br>The crRNA is composed of the sequence of the target gene and an upstream short nucleotide sequence which is complementary to the ribosome binding site (RBS). Repression of gene expression is achieved through sequestration of the RBS by formation of a stem loop structure by the 5'-untranslated region of the crRNA. This prevents binding of the RBS by the 30s ribosomal subunit and subsequent translation.
 <br> For more information about the LasR-LVA, see https://parts.igem.org/wiki/index.php?title=Part:BBa_K607002
 <br> For more information about the Prm promotor, see: https://parts.igem.org/wiki/index.phptitle=Part:BBa_K607003title=Part:BBa_K607003