Difference between revisions of "Part:BBa K546002:Design"

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===Design Notes===
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The original R0063 BioBrick part (promoter) of the F2621 BioBrick device (R0063-B0034-C0062-B0010-B0012-R0062) turned out to be absent in our construct, as was shown by a sequence analysis. To increase the transcription activation, this K546000 composite BioBrick part now contains an improved malfunctional (version of) the F2621 BioBrick device: K546003.
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===Source===
 
===Source===
  
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The lux p promoters originate from the quorum sensing ability of ''Vibrio fischeri''.
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The lux p promoters and the LuxR and LuxI proteins originate from the quorum sensing system of ''Vibrio fischeri''.
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AiiA originates from ''Bacillus sp. 240B1''.
  
 
===References===
 
===References===

Latest revision as of 19:29, 21 September 2011

Lux pL controlled luxR with lux pR controlled GFP(lva tag).


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1101
    Illegal BsaI.rc site found at 1828



Source

The lux p promoters and the LuxR and LuxI proteins originate from the quorum sensing system of Vibrio fischeri.

AiiA originates from Bacillus sp. 240B1.

References