Difference between revisions of "Part:BBa K546000:Design"

 
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<partinfo>BBa_K546000 short</partinfo>
 
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===Design Notes===
 
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The original R0063 BioBrick part (promoter) of the F2621 BioBrick device turned out to be absent in our construct, as was shown by a sequence analysis. To increase the transcription activation there has been put an extra R0063 BioBrick part promoter in front of the F2621 BioBrick device. The resulting construct worked a lot better than the original F2621 - K082014 BioBrick system.
 
  
  
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===Source===
  
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===Source===
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The lux p promoters and the LuxR and LuxI proteins originate from the quorum sensing system of ''Vibrio fischeri''.
  
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The lux p promoters and the autoinducer producing enzyme originate from the quorum sensing ability of ''Vibrio fischeri''.  
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AiiA originates from ''Bacillus sp. 240B1''.
  
 
===References===
 
===References===

Latest revision as of 19:27, 21 September 2011

Lux pL controlled LuxR with lux pR autoinducing LuxI(lva tag)- AHL.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1821
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1101



Source

The lux p promoters and the LuxR and LuxI proteins originate from the quorum sensing system of Vibrio fischeri.

AiiA originates from Bacillus sp. 240B1.

References