Difference between revisions of "Part:BBa K546546:Design"

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===Source===
 
===Source===
  
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The lux pR promoter originates from the quorum sensing ability of ''Vibrio fischeri''.
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The lux p promoters and the LuxI and LuxR proteins originate from the quorum sensing system of ''Vibrio fischeri''.
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AiiA originates from Bacillus sp. 240B1.
  
 
===References===
 
===References===

Latest revision as of 19:26, 21 September 2011

The Synchroscillator: A Tunable Synchronized Oscillatory System


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2781
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1184
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 985
    Illegal BsaI.rc site found at 2042
    Illegal BsaI.rc site found at 2930
    Illegal BsaI.rc site found at 3657


Design Notes

Some parameters of the signalling molecule formation and degradation were unknown. Modeling of the BioBrick device was therefore hard. The hybrid promoters were installed to stablize the oscillation in case it would be necessary.

Source

The lux p promoters and the LuxI and LuxR proteins originate from the quorum sensing system of Vibrio fischeri. AiiA originates from Bacillus sp. 240B1.

References