Difference between revisions of "Part:BBa K546001"

Revision as of 19:03, 21 September 2011

Lux pL controlled luxR with lux pR controlled AHL degrading enzyme AiiA(lva tag).

This device has two protein generators: The first generator ‘luxpL-RBS-C0062-B0015’ produces an intermediate level of the LuxR protein.

The second generator ‘luxpR-RBS-C0060-B0015’ has a low basal expression of the AiiA enzyme. AiiA is capable of degrading the quorum sensing molecule AHL.

It should be noted that if AHL is added to this system it forms a complex with LuxR which will increase transcription of AiiA while reducing the transcription of LuxR.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 1283
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1101

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K546001 short</partinfo>
-The F2621 BioBrick device contains a lux pL regulated luxR repressor/activator and a lux pR promoter that is linked to an 3OC6HSL, an AHL autoinducer molecule, synthetase. The lux pL promoter yields a weak constitutive downstream expression. When LuxR and 3OC6HSL are produced, the lux pL promoter, however, will be repressed by a complex of the two. If 3OC6HSL is present in the cell, the autoinducer inactivation enzyme A (''aiiA''), with an LVA Tag, will inactivate the 3OC6HSL autoinducer molecules.
+This device has two protein generators:
+The first generator  ‘luxpL-RBS-C0062-B0015’ produces an intermediate level of the LuxR protein.
+The second generator  ‘luxpR-RBS-C0060-B0015’ has a low basal expression of the AiiA enzyme. AiiA is capable of degrading the quorum sensing molecule AHL.
+It should be noted that if AHL is added to this system it forms a complex with LuxR which will increase transcription of AiiA while reducing the transcription of LuxR.
 <!-- Add more about the biology of this part here