Difference between revisions of "Part:BBa K546002"
m |
RubenvanHeck (Talk | contribs) m |
||
Line 2: | Line 2: | ||
<partinfo>BBa_K546002 short</partinfo> | <partinfo>BBa_K546002 short</partinfo> | ||
− | This | + | This device consists of two protein generators. |
+ | The first generator ‘luxpL-RBS-C0062-B0015’ produces an intermediate level of the LuxR protein. | ||
+ | The second generator ‘luxpR-RBS-J04031-B0015’has a low basal expression of the GFP, which can - of course - be monitored. | ||
+ | It should be noted that if AHL is added to this system it forms a complex with LuxR which will increase transcription of GFP while reducing the transcription of LuxR. | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Revision as of 18:57, 21 September 2011
Lux pL controlled luxR with lux pR controlled GFP(lva tag).
This device consists of two protein generators. The first generator ‘luxpL-RBS-C0062-B0015’ produces an intermediate level of the LuxR protein. The second generator ‘luxpR-RBS-J04031-B0015’has a low basal expression of the GFP, which can - of course - be monitored.
It should be noted that if AHL is added to this system it forms a complex with LuxR which will increase transcription of GFP while reducing the transcription of LuxR.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1101
Illegal BsaI.rc site found at 1828