Difference between revisions of "Part:BBa K568005:Design"

(→‎Source)
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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Red light sensor (<html><a href="https://parts.igem.org/Part:BBa_K568000">BBa_K568000</a></html>) added to intermediated part of optogenetic AND gate (<html><a href="https://parts.igem.org/Part:BBa_K568006">BBa_K568006</a></html>).
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This part was constructed in order to add T7ptag (<html><a href="https://parts.igem.org Part:BBa_K228000">BBa_K228000</a></html> at 3' site of intermediate construct in a next step.
−
Next step is cloning of T7ptag (<html><a href="https://parts.igem.org Part:BBa_K228000">BBa_K228000</a></html> at 3' site of intermediate construct.
+
  
 
===Source===
 
===Source===

Revision as of 18:46, 21 September 2011

Intermediate optogenetical AND-Gate without T7ptag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 644
    Illegal XhoI site found at 1786
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 971
    Illegal AgeI site found at 3817
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3820


Design Notes

This part was constructed in order to add T7ptag (BBa_K228000 at 3' site of intermediate construct in a next step.

Source

(BBa_K568000) (BBa_K568006)

References