Difference between revisions of "Part:BBa K568000:Design"

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===Design Notes===
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===Source===
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PCR product on relevant part of BBa_K322127
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This part was extracted from the original part <html><a href="https://parts.igem.org/Part:BBa_K322127">BBa_K322127</a></html> by PCR.
  
 
===Sequence===
 
===Sequence===

Latest revision as of 18:42, 21 September 2011

Red light sensor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 644
    Illegal XhoI site found at 1786
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 971
  • 1000
    COMPATIBLE WITH RFC[1000]


Source

This part was extracted from the original part BBa_K322127 by PCR.

Sequence

Sequence of first 1000 bp of PCR-Product shows one possible silent mutation in PcyA (Phycocyanobilin:ferredoxin oxidoreductase) gen on Position 45 T->C. Resulting triplet is CCC instead of CCT, which both code for proline.


References

[1] G A Gambetta and J C Lagarias, "Genetic engineering of phytochrome biosynthesis in bacteria." Proc Natl Acad Sci U S A 98(19):10566-71 (2001) PMID 11553807