Difference between revisions of "Part:BBa K607000"

Revision as of 18:13, 21 September 2011

PhybB_taRNA

taRNA under the control of the hybB promoter. In the AND gate of our system, repression of gene expression is achieved through sequestration of the RBS by formation of a stem loop structure by the 5'-untranslated region of the crRNA. This prevents binding of the RBS by the 30s ribosomal subunit and subsequent translation. The taRNA in this part exhibits partial complementarity to the cis-repressive sequence. Its expression, which is driven by this hybB promoter, allows formation of a RNA duplex molecule and is accompanied by a conformational change in the crRNA structure. The stem-loop structure is unfolded, subsequently abolishing RBS obstruction and allowing translation of the target gene. Reference: Isaacs at al. in 2004

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 399

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K607000 short</partinfo>
-taRNA under the control of the hybB promoter. In the AND gate, repression of gene expression is achieved through sequestration of the RBS by formation of a stem loop structure by the 5'-untranslated region of the crRNA. This prevents binding of the RBS by the 30s ribosomal subunit and subsequent translation.
+taRNA under the control of the hybB promoter. In the AND gate of our system, repression of gene expression is achieved through sequestration of the RBS by formation of a stem loop structure by the 5'-untranslated region of the crRNA. This prevents binding of the RBS by the 30s ribosomal subunit and subsequent translation.
-The taRNA exhibits partial complementarity to the cis-repressive sequence. Its expression, which is driven by a second promoter, allows formation of a RNA duplex molecule and is accompanied by a conformational change in the crRNA structure. The stem-loop structure is unfolded, subsequently abolishing RBS obstruction and allowing translation of the target gene.
+The taRNA in this part exhibits partial complementarity to the cis-repressive sequence. Its expression, which is driven by this hybB promoter, allows formation of a RNA duplex molecule and is accompanied by a conformational change in the crRNA structure. The stem-loop structure is unfolded, subsequently abolishing RBS obstruction and allowing translation of the target gene.
 Reference: Isaacs at al. in 2004