Difference between revisions of "Part:BBa K613013"

Revision as of 17:43, 21 September 2011

TetR V36F mutant

TetR with a point mutation V36F.

In vitro characterization

à toi lilia :)

In vivo characterization

This TetR mutant was characterized in vivo by putting it into pSB3K1 under a constitutive promoter (J23116). This plasmid was cotransformed with https://parts.igem.org/Part:J61002 J61002] harbouring RFP under pTet promoter (B0040) in DH5alpha cells. Cells were grown in a medium containing Kanamycin & Amplicillin plus different concentrations of ATC, ranging from 0 to 2000 ng/mL. OD600 and RFP fluorescence were measured every 10 minutes during 12 hours on a platereader machine.

Induction curves

The RFUs have been normalized by OD600 to have a better comparison between the different ATC concentrations.

Dose-response curve

For each ATC concentration, RFUs have been first divided by the corresponding OD600 value. Then, the last 6 values have been averaged to get the plateau value.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 4: / Line 4: @@
 TetR with a point mutation V36F.
-<!-- Add more about the biology of this part here
-===Usage and Biology===
+===In vitro characterization===
+à toi lilia :)
+===In vivo characterization===
+This TetR mutant was characterized ''in vivo'' by putting it into [https://parts.igem.org/Part:pSB3K1 pSB3K1] under a constitutive promoter ([https://parts.igem.org/Part:J23116 J23116]). This plasmid was cotransformed with https://parts.igem.org/Part:J61002 J61002] harbouring RFP under pTet promoter ([https://parts.igem.org/Part:B0040 B0040]) in DH5alpha cells. Cells were grown in a medium containing Kanamycin & Amplicillin plus different concentrations of ATC, ranging from 0 to 2000 ng/mL. OD600 and RFP fluorescence were measured every 10 minutes during 12 hours on a platereader machine.
+'''Induction curves'''
+The RFUs have been normalized by OD600 to have a better comparison between the different ATC concentrations.
+'''Dose-response curve'''
+For each ATC concentration, RFUs have been first divided by the corresponding OD600 value. Then, the last 6 values have been averaged to get the plateau value.
 <!-- -->