Difference between revisions of "Part:BBa K568000:Design"
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===Design Notes=== | ===Design Notes=== | ||
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===Sequence=== | ===Sequence=== | ||
Revision as of 17:24, 21 September 2011
Red light sensor
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 644
Illegal XhoI site found at 1786 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 971
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
PCR product on relevant part of BBa_K322127
Sequence
Sequence of first 1000 bp of PCR-Product shows one possible silent mutation in PcyA (Phycocyanobilin:ferredoxin oxidoreductase) gen on Position 45 T->C. Resulting triplet is CCC instead of CCT, which both code for proline.
References
[1] G A Gambetta and J C Lagarias, "Genetic engineering of phytochrome biosynthesis in bacteria." Proc Natl Acad Sci U S A 98(19):10566-71 (2001) PMID 11553807