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| | ===Applications of BBa_K676013=== | | ===Applications of BBa_K676013=== |
| − | [[Image:spy.jpg]]
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| − | According to our result in the 1D chloroquine gel electrophoresis (at 2.5 μg/mL), the plasmid with the Mu phage (pSB1C3 backbone with MuGBS/spy device - BBa_K676013) migrated further compared to the control plasmid (pSB1C3 with just spy device BBa_K239009). At the chloroquine concentration which we used, the more negatively supercoiled plasmid will migrate further while the more relaxed molecules will travel slower through the gel.
| + | [[Image:Improving_BBa_K239009_into_BBa_K676013.jpg]] |
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| − | So this indicate that the presence of the Mu phage GBS on the plasmid increase the consistency and level of supercoiling in the plasmid when compared to the control. Although this study needs to be repeated using plasmid backbones with equal length for better comparison.
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| − | [[Image:Fluorescence_Assay.jpg]] | + | |
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| − | We also set up 24 hours shake flask culture using cells with BBa_K676013 (on pSB1A3) and cells with BBa_K239009 (on pSB1K3) to compare the level of expression of GFP between the presence and absence of the Mu phage GBS sequence on plasmid. There seems to be a clear increase in the expression of GFP (and therefore the stress response) in the presence of the Mu phage GBS sequence on the plasmid.
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| | ===User Reviews=== | | ===User Reviews=== |
Revision as of 17:22, 21 September 2011
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Applications of BBa_K676013
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