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| | ===Applications of BBa_K676012=== | | ===Applications of BBa_K676012=== |
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| − | According to our result in the 1D chloroquine gel electrophoresis (at 2.5 μg/mL), the plasmid with the pBR322 GBS (on pSB1C3 backbone) did not migrate farther compared to the control plasmid (pSB1C3 with RFP expression cassette - BBa_J04450). At the chloroquine concentration which we used, the more negatively supercoiled plasmid will migrate further while the more relaxed molecules will travel slower through the gel.
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| − | Therefore this gel indicate that the pBR322 despite being a gyrase binding site has either very weak affinity for gyrase or is not good at introducing supercoils following the association with gyrase.
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| − | Besides achieving a higher level of supercoiling, the quality of the supercoiled plasmids also showed to be better. There seems to be a 34% reduction in the level of maximum supercoiling in the plasmid with pBR322 when compared to the control. Also there is a reduction in the consistency of supercoiling with the pBR322 GBS as displayed by the wider range of the band for the sample.
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| | ===User Reviews=== | | ===User Reviews=== |
Latest revision as of 17:20, 21 September 2011
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Applications of BBa_K676012
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