Difference between revisions of "Part:BBa K638001"

Revision as of 17:01, 21 September 2011

Reflectin A1 from Loligo

This is the coding region for reflectin protein A1 (originally found in the mantle of squid Loligo pealeii) codon optimised for E. coli. [http://2011.igem.org/Team:Cambridge Cambridge 2011] overexpressed it for purification using the his-tagged generator BBa_K638201 . We also exported it as a TorA fusion.

Usage and Biology

In the squid this protein is believed to self assemble with membranes to form iridophore platelets - stacks of protein ultrastructure evenly spaced to form a Bragg stack. This creates living structural colour from thin film interference effects. In L. pealeii reflectins are phosphorylated, with [http://www.ncbi.nlm.nih.gov/pubmed/19776150 changes in phosphorylation status correlating with changes in tissue colour]. Read more about [http://2011.igem.org/Team:Cambridge/Project/Background the background to this protein].

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 22
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 ===Usage and Biology===
-In the squid this protein is believed to self assemble with membranes to form iridophore platelets - stacks of protein ultrastructure evenly spaced to form a Bragg stack.  This creates living structural colour.  In ''L. pealeii reflectins are phosphorylated, which changes in phosphorylation status correlating with changes in colours created by thin film interference.  [http://2011.igem.org/Team:Cambridge/Project/Background Read more about the background to this protein].
+In the squid this protein is believed to self assemble with membranes to form iridophore platelets - stacks of protein ultrastructure evenly spaced to form a Bragg stack.  This creates living structural colour from thin film interference effects.  In ''L. pealeii'' reflectins are phosphorylated, with [http://www.ncbi.nlm.nih.gov/pubmed/19776150 changes in phosphorylation status correlating with changes in tissue colour].  Read more about [http://2011.igem.org/Team:Cambridge/Project/Background the background to this protein].
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