Difference between revisions of "Part:BBa K515104"

Revision as of 16:42, 21 September 2011

J23100 promoter - Antiholin

A composite part between J23100 and anti-holin (BBa_K515004). The promoter has been chosen in order to comply with our Gene Guard device specifications. It is under the influence of a strong promoter due to the lower copy number of this gene (will be integrated into the genome) in comparison to that of the Holin gene which will be in a high copy number plasmid within our system.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 276

This BioBrick has been sequence verified.

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-A composite part between J23100 and Anti-holin <a href="https://parts.igem.org/Part:BBa_K515004:Design">(BBa_K515004)</a>. The promoter has been chosen in order to comply with our Gene Guard specifications. It is under the influence of a strong promoter due to the lower copy number of this gene (will be integrated into the genome) in comparison to that of the Holin gene which will be in a high copy number plasmid within our system.
+A composite part between J23100 and anti-holin <a href="https://parts.igem.org/Part:BBa_K515004:Design">(BBa_K515004)</a>. The promoter has been chosen in order to comply with our Gene Guard device specifications. It is under the influence of a strong promoter due to the lower copy number of this gene (will be integrated into the genome) in comparison to that of the Holin gene which will be in a high copy number plasmid within our system.
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