Difference between revisions of "Part:BBa K608151"
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K608151 short</partinfo>
 
<partinfo>BBa_K608151 short</partinfo>
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[[Image:Enz.jpg|thumb|300px|scheme of enzyme ho1 and pcyA involved in chromophore production]]
  
 
We designed this composite in order to gain the enzyme ''pcyA'' (phycocyanobilin-ferredoxin oxidoreductase),<br/>
 
We designed this composite in order to gain the enzyme ''pcyA'' (phycocyanobilin-ferredoxin oxidoreductase),<br/>
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this design is essential if the part is going be integrated into an assembly of various genes.<br/>
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this design is necessary if the part is going be integrated into an assembly of various genes.<br/>
 
The enzyme plays an important role in the production of the PCB chromophore (phycocyanobilin)<br/>
 
The enzyme plays an important role in the production of the PCB chromophore (phycocyanobilin)<br/>
 
which is essential for the green and red light receptor system.<br/>
 
which is essential for the green and red light receptor system.<br/>
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==Usage and Biology==
 
==Usage and Biology==
  
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For PCB chromophore production the enzyme ho1 [https://parts.igem.org/Part:BBa_I15008 ho1] (heme oxygenase)<br/> in a similar design is necessary infront of this part<br/>
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For PCB chromophore production the enzyme [https://parts.igem.org/Part:BBa_I15008 ho1] (heme oxygenase)<br/> in a similar design is necessary infront of this part<br/>
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because both enzymes are necessary to convert heme into PCB chromophore.<br/>
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because both enzymes are needed to convert heme into PCB chromophore.<br/>
  
  
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<partinfo>BBa_K608151 parameters</partinfo>
 
<partinfo>BBa_K608151 parameters</partinfo>
 
<!-- -->
 
<!-- -->
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----
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'''references'''
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Ken Okada
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"HO1 and PcyA proteins involved in phycobilin biosynthesis form a 1:2 complex with ferredoxin-1 required for photosynthesis"<br/>
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FEBS letters, Volume 583, Issue 8, 17 April 2009, Pages 1251-1256

Latest revision as of 16:40, 21 September 2011

translational unit of pcyA

scheme of enzyme ho1 and pcyA involved in chromophore production

We designed this composite in order to gain the enzyme pcyA (phycocyanobilin-ferredoxin oxidoreductase),
this design is necessary if the part is going be integrated into an assembly of various genes.
The enzyme plays an important role in the production of the PCB chromophore (phycocyanobilin)
which is essential for the green and red light receptor system.
Our sequencing confirmed that the part is correct in the pSB1C3 vector.
All physical DNA samples used for this composite come from the registry distribution.


Usage and Biology

For PCB chromophore production the enzyme ho1 (heme oxygenase)
in a similar design is necessary infront of this part
because both enzymes are needed to convert heme into PCB chromophore.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 830
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 383
  • 1000
    COMPATIBLE WITH RFC[1000]


references

Ken Okada "HO1 and PcyA proteins involved in phycobilin biosynthesis form a 1:2 complex with ferredoxin-1 required for photosynthesis"
FEBS letters, Volume 583, Issue 8, 17 April 2009, Pages 1251-1256