Difference between revisions of "Part:BBa K627012:Design"

Latest revision as of 16:34, 21 September 2011

Fusion of TorA sig-seq, TEV protease cleavage site and b-lactamase

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 117
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 143
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 796

Design Notes

This biobrick was built by PCR using the following PCR primers:

p_TorA-f: CTTCTAGATGAACAATAACGATCTCTTTCAGGCATC
o_Bla_igem_r: CTACTAGTATTAACCGGTCCAATGCTTAATCAGTGAGGCAC

Source

TorA signal sequence and the lactamase was amplified via PCR from pJC354, which originates from two iGEM compatible BioBricks: BBa_K208005 and BBa_I757010. The cleavage site was created, based on the infoarmations of the Brenda enzymes database, via 2 oligonucleotides ordered form SigmaAldtrich.

References

ssTorA - https://parts.igem.org/Part:BBa_K208005
blaFL - https://parts.igem.org/Part:BBa_I757010

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K627012 short</partinfo>
@@ Line 7: / Line 6: @@
 ===Design Notes===
+This biobrick was built by PCR using the following PCR primers:<br>
+* p_TorA-f: CTTCTAGATGAACAATAACGATCTCTTTCAGGCATC
+* o_Bla_igem_r: CTACTAGTATTAACCGGTCCAATGCTTAATCAGTGAGGCAC
 ===Source===
+TorA signal sequence and the lactamase was amplified via PCR from pJC354, which originates from two iGEM compatible BioBricks: BBa_K208005 and BBa_I757010. The cleavage site was created, based on the infoarmations of  the Brenda enzymes database, via 2 oligonucleotides ordered form SigmaAldtrich.
 ===References===
+ssTorA - https://parts.igem.org/Part:BBa_K208005<br>
+blaFL - https://parts.igem.org/Part:BBa_I757010<br>