Difference between revisions of "Part:BBa K608351:Experience"

 
(Applications of BBa_K608351)
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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===Applications of BBa_K608351===
 
===Applications of BBa_K608351===
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We managed to perform a last-minute, "blind" -ie not knowing if the sequence of the cloning was correct-, and preliminary OD-measurement testing of the part in conjuction with BBa_K608352, as part of our temperature sensitive phage lysis cassette.
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<br>
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We later verified the part sequence. Please refer to our Project Description Page (http://2011.igem.org/Team:Freiburg/Description#The_temperature_sensitive_lysis_cassette) for more Info.
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<br>
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The OD values of the measurement are shown in the chart (please do bear in mind that this was a measurement done under deadline-stress, without the possibility to plan and perform something more concrete before the Wiki-Freeze)
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<br>
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:LB with Cm (Chloramphenicol) was used as a blank before every (hourly) measurement
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[[Image:Freiburg11_LysisCassetteODmeasurement.jpg|900px]]
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Nr1: Insert is only this part (BBa_K608351)
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Nr2: Insert is only BBa_K608352
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Nr3: Both parts were correctly assembled (1:BBa_K608351 + 2:BBa_K608352)
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Therefore BBa_K608351 seems to be functioning, though not tightly. More tests are planned if time allows (ie wiki-unfreeze ;) ) One sure thing is that the correct testing would be done with GFP or RFP instead of BBa_K608352, and also at different temperatures (probably a 25°C to 42°C gradient).
  
 
===User Reviews===
 
===User Reviews===

Revision as of 15:27, 21 September 2011

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K608351

We managed to perform a last-minute, "blind" -ie not knowing if the sequence of the cloning was correct-, and preliminary OD-measurement testing of the part in conjuction with BBa_K608352, as part of our temperature sensitive phage lysis cassette.


We later verified the part sequence. Please refer to our Project Description Page (http://2011.igem.org/Team:Freiburg/Description#The_temperature_sensitive_lysis_cassette) for more Info.


The OD values of the measurement are shown in the chart (please do bear in mind that this was a measurement done under deadline-stress, without the possibility to plan and perform something more concrete before the Wiki-Freeze)

LB with Cm (Chloramphenicol) was used as a blank before every (hourly) measurement


Freiburg11 LysisCassetteODmeasurement.jpg


Nr1: Insert is only this part (BBa_K608351)
Nr2: Insert is only BBa_K608352
Nr3: Both parts were correctly assembled (1:BBa_K608351 + 2:BBa_K608352)


Therefore BBa_K608351 seems to be functioning, though not tightly. More tests are planned if time allows (ie wiki-unfreeze ;) ) One sure thing is that the correct testing would be done with GFP or RFP instead of BBa_K608352, and also at different temperatures (probably a 25°C to 42°C gradient).

User Reviews

UNIQ6779d12d4b80e82d-partinfo-00000000-QINU UNIQ6779d12d4b80e82d-partinfo-00000001-QINU