Difference between revisions of "Part:BBa K608352"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | + | The phage lysis cassette consists of the standard bacteriophage λ lysis genes S/S’, R and Rz/Rz1, coded in overlapping sequences with phase 1 and 2 frameshifts. | |
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The phage λ lysozyme (R Endolysin) hydrolyses the beta-1,4-glycosidic bond between N-acetylmuramic acid (MurNAc) and N-acetylglucosamine (GlcNAc), but in order to degrade the cell wall it requires a small transmembrane protein called holin which perforates the membrane for endolysin to gain access to the murein [Ry Young et al]. The auxiliary lysis proteins Rz and Rz1 have long been known to play a vital role but have yet to be assigned a specific function stemming from functional and structural analysis. The assumption is that they form a complex spanning the periplasm and fuse the outer and inner membranes, removing the last physical barrier for cell lysis [Joel Berry et al]. | The phage λ lysozyme (R Endolysin) hydrolyses the beta-1,4-glycosidic bond between N-acetylmuramic acid (MurNAc) and N-acetylglucosamine (GlcNAc), but in order to degrade the cell wall it requires a small transmembrane protein called holin which perforates the membrane for endolysin to gain access to the murein [Ry Young et al]. The auxiliary lysis proteins Rz and Rz1 have long been known to play a vital role but have yet to be assigned a specific function stemming from functional and structural analysis. The assumption is that they form a complex spanning the periplasm and fuse the outer and inner membranes, removing the last physical barrier for cell lysis [Joel Berry et al]. | ||
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[[Image:Freigem11 Presentation3.jpg|500px]] | [[Image:Freigem11 Presentation3.jpg|500px]] | ||
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<br> | <br> | ||
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| + | ====References==== | ||
| + | Ry Young et al<br/> | ||
| + | “Phages will out: strategies of host cell lysis”<br/> | ||
| + | Trends in Microbiology Vol 8, Issue 3 (2000) 120-128<br/><br/> | ||
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| + | Joel Berry et al<br/> | ||
| + | “The final step in the phage infection cycle: the Rz and Rz1 lysis proteins link the inner and outer membranes”<br/> | ||
| + | Molecular Microbiology 70 (2008) 341–351<br/><br/> | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Revision as of 14:48, 21 September 2011
Bacteriophage Lysis Cassette with RBS
Bacteriophage Lysis cassette based on the part BBa_K124017 (note the overlapping coding regions of the sequence in spite of the given sequence for BBa_K124017) with an additional strong RBS (BBa_B0034)
Usage and Biology
The phage lysis cassette consists of the standard bacteriophage λ lysis genes S/S’, R and Rz/Rz1, coded in overlapping sequences with phase 1 and 2 frameshifts.
- S: λ Anti-Holin
- S’: λ Holin
- R: λ Endolysin
- Rz: Putative type-II signal anchor protein
- Rz1: Outer membrane lipoprotein
The phage λ lysozyme (R Endolysin) hydrolyses the beta-1,4-glycosidic bond between N-acetylmuramic acid (MurNAc) and N-acetylglucosamine (GlcNAc), but in order to degrade the cell wall it requires a small transmembrane protein called holin which perforates the membrane for endolysin to gain access to the murein [Ry Young et al]. The auxiliary lysis proteins Rz and Rz1 have long been known to play a vital role but have yet to be assigned a specific function stemming from functional and structural analysis. The assumption is that they form a complex spanning the periplasm and fuse the outer and inner membranes, removing the last physical barrier for cell lysis [Joel Berry et al].
References
Ry Young et al
“Phages will out: strategies of host cell lysis”
Trends in Microbiology Vol 8, Issue 3 (2000) 120-128
Joel Berry et al
“The final step in the phage infection cycle: the Rz and Rz1 lysis proteins link the inner and outer membranes”
Molecular Microbiology 70 (2008) 341–351
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]