Difference between revisions of "Part:BBa K627011"
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<partinfo>BBa_K627011 short</partinfo>
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This construct contains the cleavage site for the 14_3C protease flanked by the sequence of beta-lactamase and the TorA signal sequence for the TAT (twin arginine transporter) pathway, thus in case of the expression of the complete construct the beta lactamase can be transported into periplasm and provide ampicillin resistance. The TorA signal-sequence originate from the enzyme Trimethylamin-N-Oxid-Reduktase , which needs to be folded in the cytoplasm. The beta lactamase is an enzyme which cleaves lactam rings and makes bacteria resistant to antibiotics like ampicillin and penicillin. The cleavage site for the HRV 14 3C protease was created via oligo hybridization and ligated into the construct with cleavage site for XhoI and NheI, which makes this part modular and easy adaptable for any other protease.
 
This construct contains the cleavage site for the 14_3C protease flanked by the sequence of beta-lactamase and the TorA signal sequence for the TAT (twin arginine transporter) pathway, thus in case of the expression of the complete construct the beta lactamase can be transported into periplasm and provide ampicillin resistance. The TorA signal-sequence originate from the enzyme Trimethylamin-N-Oxid-Reduktase , which needs to be folded in the cytoplasm. The beta lactamase is an enzyme which cleaves lactam rings and makes bacteria resistant to antibiotics like ampicillin and penicillin. The cleavage site for the HRV 14 3C protease was created via oligo hybridization and ligated into the construct with cleavage site for XhoI and NheI, which makes this part modular and easy adaptable for any other protease.
 
   
 
   

Revision as of 14:44, 21 September 2011

Fusion part of pBAD arabinose-inducible induction system and the HRV 14_3C protease
This construct contains the cleavage site for the 14_3C protease flanked by the sequence of beta-lactamase and the TorA signal sequence for the TAT (twin arginine transporter) pathway, thus in case of the expression of the complete construct the beta lactamase can be transported into periplasm and provide ampicillin resistance. The TorA signal-sequence originate from the enzyme Trimethylamin-N-Oxid-Reduktase , which needs to be folded in the cytoplasm. The beta lactamase is an enzyme which cleaves lactam rings and makes bacteria resistant to antibiotics like ampicillin and penicillin. The cleavage site for the HRV 14 3C protease was created via oligo hybridization and ligated into the construct with cleavage site for XhoI and NheI, which makes this part modular and easy adaptable for any other protease.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1523
    Illegal BglII site found at 1711
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1239
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961