Difference between revisions of "Part:BBa K627011:Design"

(→‎Design Notes)
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
 
This biobrick was built by PCR using the following PCR primers:<br>
 
This biobrick was built by PCR using the following PCR primers:<br>
−
Forward primer (p_TorA-f): <br>
+
Forward primer (p_TorA-f): CTTCTAGATGAACAATAACGATCTCTTTCAGGCATC <br>
−
CTTCTAGATGAACAATAACGATCTCTTTCAGGCATC <br>
+
Reverse primer (o_bla_igem_r): CTACTAGTATTAACCGGTCCAATGCTTAATCAGTGAGGCAC
−
Reverse primer (o_bla_igem_r): <br>
+
−
CTACTAGTATTAACCGGTCCAATGCTTAATCAGTGAGGCAC
+
  
 
===Source===
 
===Source===

Revision as of 14:39, 21 September 2011

Fusion part of pBAD arabinose-inducible induction system and the HRV 14_3C protease


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1523
    Illegal BglII site found at 1711
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1239
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Design Notes

This biobrick was built by PCR using the following PCR primers:
Forward primer (p_TorA-f): CTTCTAGATGAACAATAACGATCTCTTTCAGGCATC
Reverse primer (o_bla_igem_r): CTACTAGTATTAACCGGTCCAATGCTTAATCAGTGAGGCAC

Source

References